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Fig. 3 | Plant Methods

Fig. 3

From: Efficient isolation of Magnolia protoplasts and the application to subcellular localization of MdeHSF1

Fig. 3

The protoplast transfection ratio and transient expression. The ratio of transfection at 10, 15, 20, 25 and 30% (w/v) PEG4000 concentration (a), the ratio of transfection after 5, 10, 15 and 20 min (b), images of GFP and chlorophyll autofluorescence (Chl) as well as bright field images of protoplasts captured 12 h after transfection (c, d). Bar in c, 50 μm. Bar in d, 10 μm

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Plant Methods

ISSN: 1746-4811