Fig. 6

ChIP analysis of SVP binding to the genomic region of FT in Arabidopsis mesophyll protoplasts incubated at 10 and 27 °C. a ChIP-qPCR assay of binding of SVP to the genomic regions of FT in Arabidopsis mesophyll protoplasts incubated at the indicated temperatures. The svp-32 protoplasts transfected with 35S::SVP:HA constructs were incubated at 23 °C for 2 h, and then transferred to 10 and 27 °C for overnight. The amplified regions 1, 2, and N (negative control) within FT used for qPCR experiments are shown in Fig. 2a. The ChIP results obtained from 3 independent biological replicates are represented as percentage of input (% Input). Error bars indicate the SEM. b SVP-HA protein expression in Arabidopsis protoplasts at different temperatures. Protoplasts were harvested before transfer (23 °C) and after transfer to 10and 27 °C. Western blot analysis was performed using anti-HA antibody to detect SVP-HA protein