Fig. 5

ChIP-qPCR analysis of SVP binding to the FT and SOC1 genomic regions in Arabidopsis svp and svp flm protoplasts. a Diagram of the SOC1 genomic regions. Closed boxes represent exons. The known binding site of SVP [1 (+206 to +454)] in SOC1 is shown [56, 57]. N, a negative control (+2270 to +2508 in SOC1). The amplified regions within FT used for qPCR experiments are shown in Fig. 2a. b ChIP-qPCR results that show SVP binding to the genomic regions of FT and SOC1 in svp-32 and svp-32 flm-3 protoplasts. The ChIP results obtained from 3 independent biological replicates are represented as percentage of input (% input). Error bars indicate the SEM