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Fig. 4 | Plant Methods

Fig. 4

From: A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in Arabidopsis mesophyll protoplasts

Fig. 4

Comparison of SVP binding to the FT genomic region in Arabidopsis mesophyll protoplasts and transgenic plants. a ChIP-qPCR analysis of SVP binding to the FT region. The amplified regions within FT used for qPCR experiments are shown in Fig. 2a. 35S::SVP:HA plants used in this study were previously described [18]. The ChIP results obtained from 3 independent biological replicates are represented as percentage of input (% input). Error bars indicate the SEM. b Relative binding of SVP binding to the FT region in protoplasts and transgenic plants. For two amplified regions (1 and 2 in Fig. 2a), the levels of immunoprecipitation by anti-HA antibody were normalized to those of immunoprecipitation by anti-cMyc antibody

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