Skip to main content
Fig. 3 | Plant Methods

Fig. 3

From: A fast, efficient chromatin immunoprecipitation method for studying protein-DNA binding in Arabidopsis mesophyll protoplasts

Fig. 3

ChIP-qPCR analysis to test SVP, WER, and SPL3 binding to the genomic regions of FT, GL2, and FUL, respectively. a Diagram of the GL2 and FUL genomic regions. Closed boxes represent exons. The known binding site of WER [1 (−933 to −889) in GL2, relative to the translational start codon] [44] and the known binding site of SPL3 [1 (−466 to −440) in FUL] [43] are shown. N is a region used for a negative control (+3774 to +3884 in GL2; +3322 to +3552 in FUL). The amplified regions within FT used for qPCR experiments are shown in Fig. 2a. b ChIP-qPCR assay of binding of SVP, WER, and SPL3 transcription factors to the genomic regions of FT, GL2, and FUL, respectively, using Arabidopsis mesophyll protoplasts. The ChIP results obtained from 3 independent biological replicates are represented as percentage of input (% input). Error bars indicate the SEM

Back to article page