Fig. 2

Effect of the amounts of transfected DNA and the number of protoplasts in ChIP-qPCR. a Diagram of the genomic region of FT, which contains target sequences of the SVP transcription factor. Closed boxes represent four exons of FT. The known binding sites of SVP [1 (from −1338 to −1152, relative to the translational start codon) and 2 (+159 to +416)] in FT are shown [17, 18]. N, a negative control (+3830 to +4068 in FT). b The effect of different amounts (10, 20, and 40 µg) of 35S::SVP:HA DNA and the number of protoplasts (2 × 10⁵ and 2 × 10⁷) used for transfection. ChIP-qPCR assays of SVP binding to the two target sequences of FT are shown. The ChIP results obtained from 3 independent biological replicates are represented as percentage of input (% input). Error bars indicate the standard error of the mean (SEM). Asterisks indicate values that are significantly different from c-Myc (Student’s t test, **P < 0.01, ***P < 0.001). c SVP-HA protein expression in Arabidopsis protoplasts (2 × 10⁷). Anti-HA antibody was used to detect SVP-HA protein