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Table 3 Evaluation of field data for T4, T5, T6, T8, and T9 transgenic wheat families as well as their parental non-transgenic cv. Hi-Line, for resistance against wheat rusts, leaf rust (Puccinia triticina) and yellow rust (Puccinia striiformis f. sp. tritici), and powdery mildew (Blumeria graminis) under artificial inoculation at the field of Gemmiza Research Station during the growing seasons 2009–2010, 2010–2011, 2011–2012, 2013–2014 and 2014–2015

From: Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance

Growing season

2009–2010

2010–2011

2011–2012

2013–2014

2014–2015

Generation

Family no.

(T4)

(T5)

(T6)

(T8)

(T9)

Transgenic line no.

Fungus

Lr

Yr

Pm

Lr

Yr

Pm

Lr

Yr

Pm

Lr

Yr

Pm

Lr

Yr

Pm

7

7/4

80S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

7/7

80S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

7/12

80S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

7/30

80S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

7/33

80S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

14

14/3

R

NA

0

R/0

0

0

5MR

MR/Tr

2

20MR

5MR

2

20MR

5MR

2

14/6

0

NA

0

0

0

0

0

0

0

0

0

0

0

0

0

14/10

R/0

NA

0

R/0

0

0

30MR

30MR

3

MS/MR

20MS

3

5MS

5MS

3

14/13

0

NA

0

R

R

0

30MR

30MR

3

30MR

20MR

3

30MR

5MR

3

47

47/1

R

NA

0

R

R/0

0

R

Tr/R

0

R

Tr/R

0

R/0

R

0

47/2

Tr/R

NA

0

Tr

Tr/R

0

20MS

MS/MR

3

30MS

MS/MR

3

20MS

20MS

3

47/3

R

NA

0

Tr/R

Tr

0

MS/MR

20MS

3

20MS

20MS

3

30MS

5S

3

47/5

60S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

47/6

60S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

47/7

80S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

47/8

60S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

47/9

60S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

47/10

80S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

47/11

60S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

47/12

60S

NA

8

–

–

–

–

–

–

–

–

–

–

–

–

71

71/3

MS/MR

NA

6

MS

5S

6

60S

5S

8

60S

20S

8

60S

20S

8

71/4

MS

NA

6

20S

20MR

6

60S

30MS

6

60S

30MS

6

60S

30S

8

71/8

R

NA

0

R

Tr

0

R/0

Tr/R

0

Tr/R

R

0

R

R/0

0

71/9

30S

NA

6

60S

MS/MR

6

80S

MS

6

80S

5S

8

80-S

20S

8

71/10

80S

NA

8

80S

60S

8

80S

80S

8

80S

80S

8

80S

80S

8

Hi-Line

 

80S

NA

9

80S

80S

9

80-S

80S

9

80-S

80S

9

80-S

80S

9

  1. Lr leaf rust, Yr yellow rust, Pm powdery mildew. 0 (Lr and Yr) = no infection, R = resistant, Tr = traces, MR = moderate resistant, MS = moderate susceptible and S = susceptible. NA = not applicable. 0 (Pm) = immune, no visible signs or symptoms; 1 = highly resistant, smallflecks only; 2 = resistant, chlorotic flecks evident; 3 = resistant, large flecks with chlorosis and necrosis; 4 = intermediate, mycelium and conidia barely detectable; 5 = moderately susceptible, small to moderate-sized pustules and conidia present; 6 = moderately susceptible, predominance of moderate-sized pustules and conidia present; 7 = susceptible, at least 50% of the leaf covered with large pustules and conidia; 8 = susceptible, 75–80% of the leaf covered with large pustules and conidia; and 9 = susceptible