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Table 1 Estimated chi26-transgene copy number in the T0 transgenic plants determined byqRT-PCR

From: Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance

Line no.

(Quantity) 100 target copies

Copy average ± SD

CV of copy average

Estimated copy number

CHI 7

0.93E + 03

1.27E+03 ± 4.81E+02

0.38

2

1.28E + 03

1.61E + 03

CHI 14

0.82E + 03

0.95E+03 ± 1.80E+02

0.19

1

0.94E + 03

1.08E + 03

1.55E + 03

1.43E + 03

CHI 47

0.64E + 03

0.78E+03 ± 1.98E+02

0.25

1

0.8E + 03

0.90E + 03

CHI 71

1.43E + 03

1.45E+03 ± 1.56E+02

0.11

2

1.35E + 03

1.57E + 03

Hi-Line (non-transgenic)

–

–

–

–

–

–

  1. SD standard deviation, CV coefficient of variance