Fig. 3

Evaluation of zinc uptake kinetics in non-dwarf rice plants using the ⁶⁵Zn imaging data. a Photograph of test plants in the acrylic root box. The dotted rectangle indicates the field of view of the PETIS. b Serial images of ⁶⁵Zn uptake by rice plants fed with different initial zinc concentrations (left to right: 0.1, 0.25, 0.5, 1, 2.5, and 5 µM). The yellow rectangles indicate the regions examined for the time course analysis. c Time course of the zinc concentration in the solution for the different solutions. d Scatter plot of the uptake velocity as a function of zinc concentration. The symbols used for each dataset correspond to the initial zinc concentrations as in c. The dotted curve represents the modified Michaelis–Menten model fit to the results