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Fig. 6 | Plant Methods

Fig. 6

From: Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas

Fig. 6

Generating a novel insertion cassette. a Diagram of insertion cassette AphVIIr which contains the 1.7-kb AphVII gene, forward splicing donor sequence, and reversed splicing donor sequence. Splicing donor sequence is indicated below. Intron/exon splicing sites, stop codons, and terminator are marked. b The AphVIIr insertion cassette generated more than 2 times motility-defect mutants as compared to insertion cassette AphVIII. c The luciferase activity of Chlamydomonas cells transformed with different insertion cassettes. Luciferase interrupted by insertion cassettes possessing splicing donor sequence displayed relatively lower activity. The pattern of splicing donor sequence (red bar) is indicated by the side of AphVII (gray bar). The cutoff of positive luciferase activity is set as 100. d The distribution of luciferase expression level in Chlamydomonas transformants cells. Insertion cassettes possessing splicing donor sequence generated less transformants displaying positive luciferase expression

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