Fig. 1
From: Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas
The optimized parental strain, primers, and size of the super pool for the insertional library. a The diagram shows the IFT46::YFP construct used to rescue the ift46-1 mutant. b Flagellar length distribution in CC-125 and HS211 cells. c Western blot results demonstrated that IFT46::YFP was expressed in the HS211 strain. d Comparing the transformation efficiency of the HS211 strain with that of wild-type CC-125 cells. e The aphVIII cassette was used to generate the insertional mutation library; the aphVIII cassette-specific primer (LGR06), targeted gene-specific forward target primers (F1–F3), and reverse target primers (R1–R3) were designed to screen mutants for the target gene. f Determination of the maximal super pool size by PCR amplification of three known insertion loci in a series of super pools