Skip to main content
Fig. 5 | Plant Methods

Fig. 5

From: A novel hairpin library-based approach to identify NBS–LRR genes required for effector-triggered hypersensitive response in Nicotiana benthamiana

Fig. 5

NRC2a/b and NRC3 are general regulator required for Pto/avrPto- and Cf-4/avr4-triggered HRs. The 47 constructs of the R gene knockout library were screened with Pto/avrPto effector as described in Fig. 4. Pre-infiltration of hp#16 and #18 was found to induce a slight reduction of the Pto/avrPto-triggered HR; however, combination of the two constructs completely cancelled the HR (left panels). Hp#16 and hp#18 were also found to reduce the Cf-4/avr4-triggered HR with a similar additive effect (right panels). Photos were taken 4 days after infiltration for Pto/avrPto and 7 days after infiltration for Cf-4/avr4

Back to article page