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Fig. 2 | Plant Methods

Fig. 2

From: A novel hairpin library-based approach to identify NBS–LRR genes required for effector-triggered hypersensitive response in Nicotiana benthamiana

Fig. 2

Hairpin library design. a A total of 281 Kmers were designed, with 202 Kmers targeting a unique gene of N. benthamiana annotated genome with 100% identity, and 79 kmers targeting two or more genes. b To produce multiple gene silencing constructs, groups of six kmers were synthesized in tandem (4 “singles” and 2 “multiple”), cloned in a Gateway-enabled pUC57 vector and eventually recombined by LR reaction into the hairpin-producing destination vector pTKO2

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Plant Methods

ISSN: 1746-4811