Recent achievements obtained by chloroplast transformation

Adem, Muhamed; Beyene, Dereje; Feyissa, Tileye

doi:10.1186/s13007-017-0179-1

Plant Methods

Table 3 Agronomic traits engineered via chloroplast genome

From: Recent achievements obtained by chloroplast transformation

Site of integration	Regulatory sequences	Transgene/s	Efficiency of expression	Enhanced traits	References
rbcL/accD	Prrn/rbcL 3′	panD	>4-fold β-alanine	Tolerance to high-temperature stress	[39]
trnI/trnA	Prrn/ggagg/psbA	tps1	>169-fold transcript	Drought tolerance: growth in 6% polyethylene glycol and rehydration after 24 days of drought	[40]
rbcL/rbcL	psbA/psbA/3′rbL	Hppd	5% TSP	Resistance to herbicide	[41]
trnI/trnA	Prrn/T7 10/rps16	Badh	93–101 μM g⁻¹ FW	Salt tolerance: carrot plants survived up to 400 mM NaCl	[42]
trnfM/trnG	atpI/rps16	Lycopene β-cyclase	0.28 mg g⁻¹ DW	Herbicide resistance and triggers conversion of lycopene	[43]
rbcL/accD	Prrn/ggagg/psbA	EPSPS/aroA	NR	Resistance to glyphosate (>5 mM)	[32]
prs14/trnG	Prrn/T7 g10/TrbcL	HTP, TCY, TMT	NR	Cold-stress tolerance and increase in vitamin E in fruit	[44]
trnV/rps7/12	Prrn/Trps16	EPSPS	>10% TSP	Resistance to the herbicide glyphosate	[45]
trnV/rps12/7	Prrn/TrbcL	b-bar1	>7% TSP	Resistance to the herbicide phosphinothricin	[46]
trnI/trnA	Prrn/psbA/psbA	phaA	14.71 β-ketothiolase mg⁻¹ FW	Engineered cytoplasmic male sterility	[47]
trnI/trnA	Prrn/T7 g 10/TpsbA	γ-TMT	>7.7% TSP	Increased salt tolerance and enhanced accumulationof ɑ-tocopherol in seeds	[48]
trnI/trnA	T7g10 or psbA	RbcS	>150-fold RbcS transcript	Restoration of RuBisCO activity in rbcS mutants	[49]
rbcL/accD	Prrn/ggagg/psbA	cry2Aa2	2–3% of TSP	Resistance to Heliothis virescens, Helicoverpa zea, and Spodoptera exigua	[50]
trnV/3′rps12	prrn T7G10/rps12	Trx f, Trx m	NR	Starch synthesis	[51]
trnI/trnA	5′psbA/3′psbA	ubiC	25% DW	250-fold higher pHBA polymer accumulation than nuclear transgenic lines	[52]
rbcL/accD	PpsbA/Trsp16	TC, γ -TMT	3 nmol h⁻¹ mg⁻¹ FW	Vitamin E accumulation in tobacco and lettuce	[53]
trnV/orf708	psbA/psbA/psbA	BicA	~0.1% TSP	CO₂ capture within leaf chloroplasts	[54]
trnV/rps12/7	Prrn/rbcL/rps16	cry1A(c)	3–5% of TSP	Resistance to larvae of Heliothis virescens, Helicoverpa zea, and Spodoptera exigua	[55]
rbcL/accD	Prrn/Trps16	CrtZ, CrtW	NR	Accumulation of astaxanthin fatty acid esters in lettuce	[56]
trnV/rps12/7	Prrn/T7gene10/rbcL	cry1Ab	NR	Resistance to caterpillar of Anticarsia gemmatalis	[57]
trnI/trnA	Prrn/Trps16	MSI-99	89.75 μg g⁻¹ FW	Resistance against rice blast fungus	[30]
trnI/trnA	Prrn/TpsbA	sporamin1, CeCPI2, and chitinase2	0.85–1% TSP	Resistance against Spodoptera litura and Spodoptera exigua leaf spot, as well as soft rot diseases	[58]
trnI/trnA	Prrn/ggagg/psbA	Bt cry2Aa2 operon	45.3% TSP	100% mortality of cotton bollworm, beet armyworm; cuboidal Bt crystals formation	[59]
trnI/trnA	Prrn/ggagg/psbA	msi-99	21–43% TSP	Resistance to in planta challenge of Aspergillus flavus, Fusarium moniliforme, Verticillium dahlia, and Colletotrichum destructivum	[60]
trnI/trnA	Prrn/ggagg/rbcL	Bt cry9Aa2	~10% of TSP	Resistance to Phthorimaea operculella	[61]
trnI/trnA	Prrn/psbA/psbA	Cpo	NR	Resistance to fungal pathogens in vitro (Fusarium verticillioides, and Verticillium dahliae) and in planta (Alternaria alternata)	[62]
trnI/trnA	5′psbA/3′psbA	PelB1, PelD2	~2.42 units mg⁻¹ FW	Resistance against Erwinia soft rot	[63]
trnI/trnA	5′psbA/3′	RC1011, PG12	17–38% TSP	Resistance to Erwinia soft rot and tobacco mosaic virus	[64]
trnI/trnA	5′psbA/3′psbA	Pta	7.1–9.2% TSP	Broad-spectrum resistance against viral/bacterial/phloem-feeding insects	[51]
trnI/trnA	5′psbA/3′psbA	Bgl-1	>160-fold enzyme	Resistance against whitefly and aphid	[65]

Back to article page

ISSN: 1746-4811

Contact us

Submission enquiries: journalsubmissions@springernature.com