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Table 2 Primers efficiency of the candidate reference genes

From: Reference genes for normalization of qPCR assays in sugarcane plants under water deficit

Gene

(µM)

Leaves

Shoot roots

E (%)*

R 2*

Mean Ct

SD

CV (%)

E (%)*

R 2*

Mean Ct

SD

CV (%)

ACT

0.8

93.4

0.9983

25.64

0.87

3.40

96.8

0.9973

25.89

1.21

4.68

GAPDH

0.8

98.7

0.9996

17.96

0.67

3.76

103.5

0.9994

22.74

0.92

4.05

TUB

0.2

92.2

0.9998

23.80

0.81

3.38

N.A.

N.A.

N.A.

N.A.

N.A.

UBQ1

0.2

104.4

0.9957

28.44

0.92

3.24

95.4

0.9966

27.61

0.86

3.13

UBQ2

0.2

95.7

0.9977

19.20

0.80

4.19

103.2

0.9989

21.90

1.44

6.57

RPL

0.8

98.7

0.9997

25.64

0.87

3.40

99.2

0.9971

21.51

1.15

5.36

25SrRNA1

0.2

93.1

0.9998

10.74

1.07

10.00

N.A.

N.A.

N.A.

N.A.

N.A.

25SrRNA2

0.2

114

0.9991

11.68

0.93

7.92

N.A.

N.A.

N.A.

N.A.

N.A.

  1. Primer concentration (µM), standard deviation (SD), co-variance (CV), amplification efficiency (E) and correlation coefficient (R 2)
  2. * qPCR efficiency (E = 10(−1/slope) − 1) and correlation coefficient (R 2) were determined by standard curve by excel data. N.A. means data not analyzed