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Table 2 Primers efficiency of the candidate reference genes

From: Reference genes for normalization of qPCR assays in sugarcane plants under water deficit

Gene (µM) Leaves Shoot roots
E (%)* R 2* Mean Ct SD CV (%) E (%)* R 2* Mean Ct SD CV (%)
ACT 0.8 93.4 0.9983 25.64 0.87 3.40 96.8 0.9973 25.89 1.21 4.68
GAPDH 0.8 98.7 0.9996 17.96 0.67 3.76 103.5 0.9994 22.74 0.92 4.05
TUB 0.2 92.2 0.9998 23.80 0.81 3.38 N.A. N.A. N.A. N.A. N.A.
UBQ1 0.2 104.4 0.9957 28.44 0.92 3.24 95.4 0.9966 27.61 0.86 3.13
UBQ2 0.2 95.7 0.9977 19.20 0.80 4.19 103.2 0.9989 21.90 1.44 6.57
RPL 0.8 98.7 0.9997 25.64 0.87 3.40 99.2 0.9971 21.51 1.15 5.36
25SrRNA1 0.2 93.1 0.9998 10.74 1.07 10.00 N.A. N.A. N.A. N.A. N.A.
25SrRNA2 0.2 114 0.9991 11.68 0.93 7.92 N.A. N.A. N.A. N.A. N.A.
  1. Primer concentration (µM), standard deviation (SD), co-variance (CV), amplification efficiency (E) and correlation coefficient (R 2)
  2. * qPCR efficiency (E = 10(−1/slope) − 1) and correlation coefficient (R 2) were determined by standard curve by excel data. N.A. means data not analyzed