Reference genes for normalization of qPCR assays in sugarcane plants under water deficit

Table 1 The gene name, accession number, gene description, primer sequences and amplicon size (bp)

Gene	Accession no.	Gene description	Primer Sequence (5′–3′)	Size (bp)	References
ACT	CA148161	Actin	F: CTCAACCCCAAGGCTAACAG R: GGCATGAGGAAGGGCATAA	195	[30]
GAPDH	CA254672	Glyceraldehyde-3phosphate dehydrogenase	F: TTGGTTTCCACTGACTTCGTT R: CTGTAGCCCCACTCGTTGT	122	[30]
TUB	CA222437	Tubulin	F: CTCCACATTCATCGGCAACTC R: TCCTCCTCTTCTTCCTCCTCG	237	[30]
UBQ1	CA094944	Ubiquitin1	F: AGCCTCAGACCAGATTCCAA R: AATCGCTGTCGAACTACTTGC	110	*
UBQ2	CA093560	Ubiquitin2	F: CTTCTTCTGTCCCTCCGATG R: TCCAACCAAACTGCTGCTC	158	*
RPL	CA127053	60S ribosomal protein L35-4	F: CTGAAGACGGAGAGGGAAAA R: GGCGAAGAGAAACTAACAC	264	[31]
25SrRNA1	CO373883 CA171131	25S ribosomal RNA	F: ATAACCGCATCAGGTCTCCAAG R: CCTCAGAGCCAATCCTTTTCC	110	[30]
25SrRNA2	BQ536525	25S ribosomal RNA	F: GCAGCCAAGCGTTCATAGC R: CCTATTGGTGGGTGAACAATCC	108	[30]

ISSN: 1746-4811