Skip to main content
Fig. 5 | Plant Methods

Fig. 5

From: Protoplast isolation prior to flow cytometry reveals clear patterns of endoreduplication in potato tubers, related species, and some starchy root crops

Fig. 5

Relationship between fluorescence intensity and particle area in leaf (a) and tuber pith (b) nuclei obtained from protoplast preparations. Populations of different C-values are circled. Other points represent debris (arrows) c–j: bright-field images obtained during ImageStream flow cytometry of leaf (c, e, g) and tuber (d, f, h, j) nuclei obtained from protoplast preparations with increasing C-values which are representative of the populations circled in a, b. i Depicts a large starch granule from the “debris” in b. Scale bar = 10 μm. Note scatterplots are meant to be illustrative and were not used to calculate relative abundances of nuclei due to lower flow rate of ImageStream cytometer

Back to article page

Plant Methods

ISSN: 1746-4811