Fig. 1
From: Ex vitro hairy root induction in detached peanut leaves for plant–nematode interaction studies
Sequential steps to obtain transgenic hairy roots from peanut detached leaves. a A. rhizogenes paste prepared for petiole inoculation. b, c Inoculation of A. rhizogenes paste in the peanut petiole by puncturing with a needle. d Diagram of the moist chamber showing the Petri dish with a cotton layer covering the moistened filter paper and a microscope slide on top of them. e Detached leaf in the moist chamber after the inoculation with A. rhizogenes paste and covered by wet cotton at the wounding region of the petiole. f Emerging hairy roots, 20 days after A. rhizogenes transformation, using the bright field and g the epifluorescent to detect eGFP. h Petiole covered by vermiculite after hairy roots emergence. i Hairy roots in peanut detached leaves, 30 days after A. rhizogenes transformation. Scale bars = 1 mm in (f, g) and = 5 mm in (i)