Fig. 2
From: A rapid and efficient method for uniform gene expression using the barley stripe mosaic virus
Expression of iLOV in wheat roots and leaves. a iLOV fluorescence images of leaves of BSMV:iLOV infected plants at 7 dpi (1–4), 21 dpi (5–8) and 25 dpi (9–10). A close-up is shown to see individual cells from the first leaves at 7 dpi using the scratching method (3) or the imbibition method (4). b iLOV fluorescence images of roots of BSMV:iLOV infected plants at 7 dpi (1–2) and 21 dpi (3–4). c Presence of iLOV region within the BSMV:iLOV RNA in leaves and roots at 7 dpi detected by RT-PCR. d Quantification of the iLOV region in leaves and roots at various days post infection by qRT-PCR. The copy number is calculated for the amount of RNA used in the qRT-PCR reaction using a calibration curve with known amounts of iLOV cDNA. Values represent mean ± SD (N = 3). Different letters indicate significant differences between groups (p < 0.05)