Fig. 4

The regulatory pathway of G protein signaling in the C/N sensing evaluated by RGB value. a Proportion of green leaves of the G protein mutants in response to nitrogen under 6% glucose. To distinguish from green and not green leaves, a threshold of was established (>15 ng/mm² = green) Experiments were repeated twice with 48 seedlings each. The curves were created through global curve fitting (sigmoid equation with four parameters) with SigmaPlot 12.5. b The chlorophyll content estimated by RGB value under moderate C/N stress (4% glucose and 6 mM nitrate, n = 40). For the box plot, solid line indicates the median and the dotted line indicates the mean value. Different lowercase letters indicate the significant differences among six genotypes (p < 0.05)