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Fig. 8 | Plant Methods

Fig. 8

From: A new kymogram-based method reveals unexpected effects of marker protein expression and spatial anisotropy of cytoskeletal dynamics in plant cell cortex

Fig. 8

Effects of various fluorophores on actin dynamics as detected using Lifeact fusion proteins. Data from cotyledons of wt seedlings, recorded for 105 s. a Image to image correlation curves for representative transformants carrying the indicated Lifeact fusion proteins. Note that for Lifeact-mRFP the correlation value becomes time-independent, and thus the assay non-informative, at approximately 50 s (arrow). Asterisks denote statistical significance of the indicated differences at time points denoted by broken lines (Tukey HSD, ** for p < 0.01). b QuACK results from the same data, with a massively overexpressing Lifeact-YFP line (Lifeact-YFP ox) added for comparison but not included in statistical calculations. Presence of differences was confirmed by the Kruskal–Wallis test with p < 0.01. Statistical significance of differences between the indicated samples determined by Wilcoxon–Mann–Whitney test with the Bonferroni correction is denoted by * for p < 0.05, ** for p < 0.01. Transect size was 20 μm. Between 50 and 70 transects per sample were measured

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Plant Methods

ISSN: 1746-4811