Fig. 4

Comparison of QuACK analysis of microtubule dynamics using varying transect size with other methods. a Microtubule stability (top) and lateral mobility (bottom) determined by QuACK from VAEM recordings of cotyledon pavement cells of wt and fh1 seedlings expressing GFP-MAP4. Eight transects per movie were measured. b Fraction of microtubules at pause and exhibiting lateral movements determined by manual tracking in videos from the same experiment. Statistical significance of differences between wt and fh1 plants as determined by pairwise Wilcoxon–Mann–Whitney test with Bonferroni correction in (a) and by Chi square test in (b) is denoted by asterisks (** for p < 0.01, * for p < 0.05). NS not significant (p > 0.05). c Image to image correlation curves from the same data (modified from [26]). Asterisks denote statistically significant difference between wt and fh1 plants at time points denoted by broken lines (Tukey HSD, ** for p < 0.01)