Fig. 3

Reproducibility of QuACK results. a Actin structure stability in cotyledon pavement cells of wt and fh1 seedlings expressing Lifeact-mRFP, determined from SDCM recordings from two independent experiments involving separate cultivations using QuACK with transect length of 20 μm. While the actual values measured for each genotype vary between experiments, the presence and direction of inter-genotype differences is reproducible. b Actin structure stability estimated as in (a) from a set videos obtained in Experiment 1, analyzed either in their original state or after controlled introduction of noise using the ImageJ “Process–Noise–Add specified noise” command with standard deviation value set at 100. Asterisks in (a) and (b) denote statistical significance at p < 0.05 (*) or p < 0.01 (**) determined by pairwise Wilcoxon–Mann–Whitney test with Bonferroni correction. NS not significant (p > 0.05). c Sample frames from an original video (top) and the same frame after noise introduction (bottom), together with representative kymograms. Grid = 5 μm. Only recordings comparable with the original (top) video but not with the noisy (bottom) one should be considered for analysis