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Fig. 2 | Plant Methods

Fig. 2

From: A new kymogram-based method reveals unexpected effects of marker protein expression and spatial anisotropy of cytoskeletal dynamics in plant cell cortex

Fig. 2

Comparison of QuACK analysis of actin dynamics using varying transect size with manual filament tracking. a Actin structure stability (top) and lateral mobility (bottom) determined by QuACK from SDCM recordings of cotyledon pavement cells of wt and fh1 seedlings expressing Lifeact-mRFP. b Microfilament pause duration (top) and fraction of laterally moving actin structures (bottom) determined by manual tracking in videos from the same experiment. Statistical significance of differences between wt and fh1 plants as determined by pairwise Wilcoxon–Mann–Whitney test (with Bonferroni correction in (a)) or by Chi square test (in the bottom panel of (b)) is denoted by asterisks (** for p < 0.01). NS not significant (p > 0.05)

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Plant Methods

ISSN: 1746-4811