Table 1 The primers used for this work
Primer | Description | Sequence 5′–3′ |
|---|---|---|
RIM-f2 | Used to amplify cassette | TGT GTG GAA TTG TGA GCG G |
RIM-r1 | Used to amplify cassette | CTT TCC ATC GGC CCA GCA |
RIM 3-1 | 3′ insert primer | CGG TAT CGG AGG AAA AGC TG |
RIM 3-2 | 3′ insert primer | GCT GTT GGA CGA GTT CTT CTG |
RIM 5-1 | 5′ insert primer | TTC CAA GCG ATC ACC AGC AC |
RIM5-2 | 5′ insert primer | GCT GGC ACG AGT ACG GGT TG |
RIM5-4 | 5′ insert primer | AGC TTT TGT TCC CTT TAG TG |
AP1 | Adaptor primer | GTA ATA CGA CTC ACT ATA GAG T |
AP2 | Adaptor primer | ACT ATA GAG TAC GCG TGG T |
RX1 | 5′ insert primer pSL18 | GCC CTC ATA GCC CGC CAA ATC AG |
RX2 | 5′ insert primer pSL18 | AAG CCG ATA AAC ACC AGC CC |