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Table 1 The primers used for this work

From: A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Primer Description Sequence 5′–3′
RIM-f2 Used to amplify cassette TGT GTG GAA TTG TGA GCG G
RIM-r1 Used to amplify cassette CTT TCC ATC GGC CCA GCA
RIM 3-1 3′ insert primer CGG TAT CGG AGG AAA AGC TG
RIM 3-2 3′ insert primer GCT GTT GGA CGA GTT CTT CTG
RIM 5-1 5′ insert primer TTC CAA GCG ATC ACC AGC AC
RIM5-2 5′ insert primer GCT GGC ACG AGT ACG GGT TG
RIM5-4 5′ insert primer AGC TTT TGT TCC CTT TAG TG
AP1 Adaptor primer GTA ATA CGA CTC ACT ATA GAG T
AP2 Adaptor primer ACT ATA GAG TAC GCG TGG T
RX1 5′ insert primer pSL18 GCC CTC ATA GCC CGC CAA ATC AG
RX2 5′ insert primer pSL18 AAG CCG ATA AAC ACC AGC CC