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Fig. 7 | Plant Methods

Fig. 7

From: A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Fig. 7

Representative results using this adaptor PCR method. Representative results from several insertional mutants (A1–A24) following the described protocol. Agarose gel electrophoresis was used to visualize the products of the primary and secondary PCR reactions respectively for each mutant. A diagnostic step-down in fragment size was indicative of a positive result as the nested primers amplified the target DNA from the mutants

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Plant Methods

ISSN: 1746-4811