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Fig. 5 | Plant Methods

Fig. 5

From: A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Fig. 5

The adaptor PCR method. A flow-chart depicting the adaptor-mediated PCR method to obtain DNA sequence flanking the insertion of the paromomycin resistance cassette (AphVIII). The bold text in the final sequence highlights the 3′ end of the insertion DNA, and the bold/italicized text depicts the 48 bp sequence that is sometimes present in shorter DNA fragments

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Plant Methods

ISSN: 1746-4811