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Fig. 4 | Plant Methods

Fig. 4

From: A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Fig. 4

The adaptor used to obtain the flanking DNA. The blunt-ended asymmetric adaptor, consisting of a 48 bp positive strand (+) and a 10 bp negative strand (−), and the two adaptor primers (AP1 and AP2) aligned to depict where they will bind after the negative strand of the adaptor is extended in the first round of the primary PCR reaction

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