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Fig. 1 | Plant Methods

Fig. 1

From: A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Fig. 1

The paromomycin cassette. Two different methods were used to generate the paromomycin cassette used in these experiments. a PCR generated cassette. The paromomycin resistance cassette used to generate paromomycin resistant strains, and the primers used to amplify the cassette (RIM-f2, and RIM-r1) from pSL72. The other primers indicated in the figure were used to amplify genomic DNA flanking the site of the insertion. b Restriction digested generated cassette. The paromomycin cassette from the pSL18 plasmid

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Plant Methods

ISSN: 1746-4811