Fig. 3
Comparisons of wheat dark respiration (R dark) measurements made using different experimental techniques. Leaf and root R dark was calculated from O2 consumption (open boxes) and CO2 evolution rates (hatched boxes), measured using fluorophore quenching, membrane inlet mass spectrometry (MIMS), Clark-type O2-electrodes, and infrared gas analysis (IRGA). Whiskers of box-plots represent the 5–95 percentile. For leaf O2 analysis, letters indicate significant differences between techniques at P < 0.05, derived from a one-way ANOVA with a Tukey’s multiple comparison test. For leaf CO2 analysis and roots an unpaired t-test was performed and * indicates significance at P < 0.01, while ***significance at P < 0.001. All measurements were made at 21 ± 0.5 °C. The values are based on six biological replicates and greater than 12 technical replicates