TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis

Table 1 Modification efficiency of the two TALEN approaches

TALEN	Custom						Assembled		Custom and assembled
Target site	TS1			TS2			TS1	TS2	TS1	TS2
Experiment	A	B	C	A	B	C
T1 plants	14	21	10	39	117	43	64	84	109	283
Mpnop1-like	10	8	2	8	26	4	4	35	24	73
Efficiency (%)	71	38	20	21	22	9	6	42	22	26

Three independent transformations were conducted for each custom TALEN target site (experiment A–C) and one for each assembled TALEN target site. In total, 392 T1 plants were analyzed. Efficiencies were determined by identification of T1 plants exhibiting the Mpnop1 knockout phenotype

ISSN: 1746-4811