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Fig. 4 | Plant Methods

Fig. 4

From: TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis

Fig. 4

Nucleotide modifications in the MpNOP1 locus induced by TALENs. Alignment of analyzed MpNOP1 TS1 and TS2 sequences from T1 mutants. The respective wild-type nucleotide and amino acid MpNOP1 sequences are depicted at the top of each target site. Numbers in brackets indicate the position of the first indicated amino acid. TALEN binding sites are underlined and spacer sequences are highlighted in grey. Origin of the TALEN pairs is indicated using C for custom TALENs (Cellectis) and A for the assembled TALENs (Sidansai) on the left side. Deletions in the genomic locus are indicated by dashes, insertions by italicized lowercase letters. Amino acid changes in out-of-frame and in-frame mutants are indicated by the suffixes ge-o and ge-i. The predicted translated protein length till occurrence of a novel, artificial stop codon in the ge-o mutants is shown on the right side along with the length of the deletions (−) and insertions (+). Asterisk indicates A40 as putative start of the U-box; nd not determined

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Plant Methods

ISSN: 1746-4811