Fig. 3

Phenotypes of TALEN-induced Mpnop1 mutations. Left panel shows 4 week-old thalli of G1 plants (a, c, g, i) and one G4 plant (e) propagated vegetatively through clonal gemmae. Boxes delineate the area chosen for close-up images depicted in b, d, f, h, j. The typical Mpnop1 kockout phenotype lacking air chamber formation is shown exemplarily for one G1 plant from the Mpnop1-4 ^ge-o line (c, d). The knockout phenotype was maintained without alterations throughout further G2 and G3 generations till G4 (e, f). Two lines with in-frame genome modifications show deviations from the typical Mpnop1 mutant phenotype by producing only exceptionally (g, h, Mpnop1-23 ^ge-i) or less frequently irregularly shaped air chambers (i, j, Mpnop1-24 ^ge-i), indicated by encirclement of one air chamber, respectively. Bars a, c, e, g, i = 5 mm; b, d, f, h, j = 1 mm