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Fig. 2 | Plant Methods

Fig. 2

From: TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis

Fig. 2

Structure of the T-DNA TALEN constructs. The left and right arm constructs of the custom TALENs (a) and assembled TALENs (b) were subcloned into pMpGWB103 and pMpGWB403 vectors, respectively. RB, LB, T-DNA left and right border; pro MpEF1α, MpEF1α promoter; attB, residual attachment site from Gateway cloning; NLS, nuclear localization signal; HA, hemagglutinin tag; S, S-peptide epitope; TALE, Transcription activator-like effectors; FokI, DNA cleavage domain of the FokI endonuclease; NOS, nopaline synthase; Ter, terminator; 35S, CaMV35S promoter; Hygr, hygromycin resistance gene; G418r, G418 resistance gene; SpeI, SacI, restriction sites for subcloning; 3×FLAG, threefold FLAG-tag

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Plant Methods

ISSN: 1746-4811