Fig. 9

Desulfoglucosinolate content extracted from B. juncea, E. sativa and R. sativus tissue incubated with 75 µl low concentration sulfatase (0.3 U/ml) over 12, 24 or 48 h, and with a high concentration sulfatase (5 U/ml) over 24 h, normalised to glucosinolate content of the same samples prior to sulfatase treatment. E. sativa leaf samples were treated with TCEP post desulfation to undimerise didesulfoglucosatavin. Asterisks indicate a significant effect of purification method on glucosinolate concentration (repeat measure ANOVA, p < 0.05). ‘d’ indicates the purification method yields a significantly different glucosinolate concentration relative to the intact glucosinolates (paired t test, p < 0.05)