Table 1 Summary of DNA extraction methods used in this study
From: Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize
DNA extraction method | Basis and format | Starting material | Extraction buffer | Elution buffer | Maximum DNA yield (ng DNA/mg sample) | DNA purity A260Â nm/A280Â nm ratio |
|---|---|---|---|---|---|---|
Qiagen | Silica membrane binding; spin-column format | 100 mg | 400 µL buffer A | 150 µL buffer A | 23.6 | 1.80–1.95 |
CTAB | Solution-based; selective precipitation of DNA | 100 mg | 1000 µL buffer (2% CTAB, 1.4 M NaCl, 20 mM EDTA, 100 mM Tris HCl pH 8.0) | 500 μL of ethanol solution (70%) | 11.2 | 1.2–2.90 |
Mericon extraction | Silica membrane binding; spin-column format | 100 mg (6 × 50 mg) | 1000 µL buffer (20 mM EDTA, 100 mM Tris HCl pH 8.0) | EB: 10 mM Tris–Cl, pH 8.5 | 386.9 | 1.61–2.00 |