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Fig. 2 | Plant Methods

Fig. 2

From: Harnessing anthocyanin-rich fruit: a visible reporter for tracing virus-induced gene silencing in pepper fruit

Fig. 2

Anthocyanin-mediated visualization of VIGS in peppers using TRV2-LIC and An2 as a reporter. a Agroinfiltration to NMH cotyledons. b Results from different tissues of TRV2-GFP-infiltrated pepper (control, left) and TRV2::GFP::An2 infiltrated pepper (right). c Expression analysis of An2 by qRT-PCR in 30 DPA fruits. Data indicate relative expression level compared to control. Three biological replicates of qRT-PCRs were performed. Data are shown as mean ± SE. Statistically significant differences, determined by Student’s t test, are indicated by an asterisk (P ≤ 0.05)

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