Fig. 4From: A DNA-based real-time PCR assay for robust growth quantification of the bacterial pathogen Pseudomonas syringae on Arabidopsis thaliana Comparative analysis of the two quantification methods for bacterial growth rates over a time course of infection. The growth of Pst DC3000 was determined by the classical colony count quantification method (I) and qRT-PCR-based biomass validation (II) over a time course of three days. The error bars indicate standard deviations of three independent biological replicates. The stars indicate statistical significance of the bacterial growth in the eds1 mutant compared to the bacterial growth in wild-type plants (WT; t test: * ≥0.05, ** ≥0.01). The results of the two assays from I and II were plotted against each other (III). The correlation coefficients are indicated (R2). All experiments were repeated at least three times with comparable resultsBack to article page