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Fig. 3 | Plant Methods

Fig. 3

From: A DNA-based real-time PCR assay for robust growth quantification of the bacterial pathogen Pseudomonas syringae on Arabidopsis thaliana

Fig. 3

Comparative analysis of the two quantification methods for bacterial growth rates upon spray inoculation of Arabidopsis leaves. a The growth of Pst DC3000 was determined by the classical colony count quantification method (I) and qRT-PCR-based biomass validation (II). The error bars indicate standard deviations of three independent biological replicates. The stars indicate statistical significance of the bacterial growth in the indicated mutants in comparison to the bacterial growth in wild-type plants (WT; t test: * ≥0.05, ** ≥0.01). The results of the two assays from I and II were plotted against each other (III). The correlation coefficient is indicated (R2). b The growth of Pseudomonas syringae DC3000 AvrRps4 was quantified as for Pst DC3000 in a. All experiments were repeated at least three times with comparable results

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