Skip to main content
Fig. 2 | Plant Methods

Fig. 2

From: Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample

Fig. 2

Schematic overview of the applied analytical methods. Following the two plus one phase extraction procedure, a phase separation of upper non-polar metabolites and a lower layer of polar to semi-polar metabolites next to a solid pellet (proteins, starch and cell wall) is obtained. A predefined volume (0.5 ml) of the upper lipid phase is aliquoted into three fractions (0.2, 0.2 and 0.1 ml), which are concentrated and analyzed by UPLC-MS, LC- photodiode array (PDA) or spectrometry for the lipid, pigment or chlorophyll composition, respectively. Two aliquots with predefined volume (0.2 and 0.4 ml) of the lower methanol: water phase are dried and the re-suspended compounds are analysed by GC- and UPLC-MS for analysis of primary and secondary metabolite composition, respectively. The starch/protein/cell wall pellet is washed followed by sequential protein and starch extraction. The de-proteinated and de-starched pellet, which contains the remaining cell wall material, can be used for determination of polysaccharide composition, cellulose and lignin

Back to article page