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Fig. 1 | Plant Methods

Fig. 1

From: Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample

Fig. 1

Overview of the experimental workflow for the MTBE-based extraction procedure. Plant material is harvested and snap-frozen in liquid nitrogen. The harvested tissue is homogenized using a pre-cooled mortar and pestle or cooled tubes in a mixer mill. About 10–50 mg ±10% of the frozen powder is weighed in pre-labelled 2 ml microcentrifuge tube. The weighed powder is extracted using 1 ml of the first extraction solvent (M1, MTBE:MeOH 3:1, vol/vol) followed by rigorous vortexing, agitated incubation and sonication of the samples. A liquid/liquid phase separation is achieved by adding 0.65 ml of the second extraction solvent (M2, H2O:MeOH 3:1, vol/vol) followed by vortexing and centrifugation

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Plant Methods

ISSN: 1746-4811