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Fig. 1 | Plant Methods

Fig. 1

From: Chlorophyll a fluorescence, under half of the adaptive growth-irradiance, for high-throughput sensing of leaf-water deficit in Arabidopsis thaliana accessions

Fig. 1

A schematic diagram of transect fluorescence platform. It consists of a motorized 3 m long arm along with a customized open version of imaging fluorometer; it can move with a precision of 1 mm and “capture” chlorophyll a fluorescence (ChlF) transients. Open version of fluorometer consists of a CCD camera, two LED panels (~620 nm) that provide both actinic light and measuring flashes, and another LED panel that gives high intensity saturating flashes. The imaging fluorometer as well as movement of camera was fully controlled by protocols through computer. Four white LED panels, each of 1.32 m × 0.32 m, were installed above the transect edge; thus, the plants can even grow right on the platform. For details of fluorometer, see Nedbal et al. [39]

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