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Table 2 Mapping of essential MIAPPE attributes to ISA-Tab structures in Phenotyping Configuration: Basic, Field and Greenhouse

From: Measures for interoperability of phenotypic data: minimum information requirements and formatting

Checklist section ISA-Tab level Checklist attribute ISA-Tab structure Basic Field Greenhouse
General metadata Investigation Unique identifier  
Timing and location Study Timing  Start of experiment (date)  Duration (days/months/year) Characteristics
Experiment location  Geographic location
Biosource Study Organism (taxon) Characteristics
Infraspecific name
Seed origin
Environment Study Growth facility (growth chamber, GC/greenhouse, GH/open top chamber, OTC/experimental garden/field) Characteristics
Aerial conditions  Air humidity (moisture)  Daily photon flux (light intensity)  Temperature (°C):   Average day temperature    Average night temperature Protocol “Aerial conditions” with parameters  
Rooting conditions  Rooting medium: aeroponics/hydroponics (water-based, solid-media based)/soil type (sand, peat, clay, mixed, etc.)  pH  For field:   Plot size   Sowing density  For greenhouse:   Container type   Container volume   Container dimensions   Number of plants per container Protocol “Rooting” with parameters  
Nutrients  For soil:   Extractable N content per unit ground area before fertiliser added   Type and amount of fertiliser added, Protocol “Nutrition” with parameters  
Watering  For soil:   Range in water potential (MPa)   Irrigation from top/bottom/drip irrigation Protocol “Watering” with parameters  
Treatments Study or Assay All interventions being part of the experiment Factor or Protocol with parameters  
Experimental design Study Experimental units and their grouping (into blocks, superblocks etc.) Characteristics, Factor, Protocol “Sampling” with parameters  
Sample collection, processing, management Assay Plant body of interest (organ) Characteristics
Observational variables Assay Phenotypic variables  Trait  Method  Scale  
Environmental variables  Trait  Method  Scale  
Observations Assay Raw data Raw data file
Derived data Derived data file
  1. —included in the ISA-Tab configuration; □—not included in the configuration, specific per experiment