Table 2 Mapping of essential MIAPPE attributes to ISA-Tab structures in Phenotyping Configuration: Basic, Field and Greenhouse

General metadata

Investigation

Unique identifier

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●

●

Title

●

●

●

Description

●

●

●

Timing and location

Study

Timing

 Start of experiment (date)

 Duration (days/months/year)

Characteristics

●

●

●

Experiment location

 Geographic location

●

●

●

Biosource

Study

Organism (taxon)

Characteristics

●

●

●

Infraspecific name

●

●

●

Seed origin

●

●

●

Environment

Study

Growth facility (growth chamber, GC/greenhouse, GH/open top chamber, OTC/experimental garden/field)

Characteristics

●

●

●

Aerial conditions

 Air humidity (moisture)

 Daily photon flux (light intensity)

 Temperature (°C):

  Average day temperature

   Average night temperature

Protocol “Aerial conditions” with parameters

●

●

Rooting conditions

 Rooting medium: aeroponics/hydroponics (water-based, solid-media based)/soil type (sand, peat, clay, mixed, etc.)

 pH

 For field:

  Plot size

  Sowing density

 For greenhouse:

  Container type

  Container volume

  Container dimensions

  Number of plants per container

Protocol “Rooting” with parameters

●

●

Nutrients

 For soil:

  Extractable N content per unit ground area before fertiliser added

  Type and amount of fertiliser added,

Protocol “Nutrition” with parameters

●

●

Watering

 For soil:

  Range in water potential (MPa)

  Irrigation from top/bottom/drip irrigation

Protocol “Watering” with parameters

●

●

Treatments

Study or Assay

All interventions being part of the experiment

Factor or Protocol with parameters

□

□

Experimental design

Study

Experimental units and their grouping (into blocks, superblocks etc.)

Characteristics, Factor, Protocol “Sampling” with parameters

●

●

Sample collection, processing, management

Assay

Plant body of interest (organ)

Characteristics

●

●

●

Observational variables

Assay

Phenotypic variables

 Trait

 Method

 Scale

●

●

●

Environmental variables

 Trait

 Method

 Scale

□

□

□

Observations

Assay

Raw data

Raw data file

□

□

□

Derived data

Derived data file

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●