Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants

Table 3 Summary of alignment statistics of sequencing data

Individual no.	Scaffolds (%)		CDS (%)		Repeats (%)
Individual no.	Read1	Read2	Read1	Read2	Read1	Read2
Protocol A
D. latiflorus-3	63.21	61.35	–	–	–	–
C. pallens	15.83	13.95	3.31	3.10	0.49	0.39
Protocol B
Oryza sativa	88.22	82.50	19.39	19.64	6.85	7.70
Zea mays	90.66	84.86	2.38	2.83	10.95	8.82
P. edulis	83.80	79.93	2.38	2.87	0.11	0.17
A. semialata	3.37	3.71	1.08	1.11	0.00	0.22
D. latiflorus-1	62.23	61.97	–	–	–	–
D. latiflorus-2	64.15	62.59	–	–	–	–
P. rubicunda	23.19	21.72	1.40	1.59	0.10	0.15
P. vivax	25.56	24.66	1.39	1.64	0.11	0.16

As CDS and repeats region were not available for D. latiflorus survey genome sequences, MiddRAD data of D. latiflorus individuals were only mapped to the assembled scaffolds

ISSN: 1746-4811