Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants

Table 1 Total number of fragments produced by in silico digestion of 23 species

Species	SbfI + EcoRI	SbfI + MlucI	NlaIII + MlucI	AvaII + MspI	EcoRI + MspI	PstI + MspI
Brassica rapa	2352	2795	3,710,380	271,860	88,220	70,512
Glycine max	7498	9006	16,747,131	803,332	306,391	162,398
Populus trichocarpa	4876	5769	7,169,615	329,150	147,647	97,378
Vitis vinifera	4994	5903	8,028,433	380,967	162,506	91,994
Brachypodium distachyon	12,306	14,912	3,237,571	535,195	106,924	166,584
Carica papaya	4592	5255	4,221,765	245,762	99,163	59,272
Physcomitrella patens	4786	5413	7,434,539	329,065	144,069	102,802
Cucumis sativus	1450	1737	3,342,214	116,754	62,340	32,319
Musa acuminata	6599	7483	5,467,255	433,922	170,591	129,105
Nelumbo nucifera	9469	10,906	10,160,790	944,853	308,661	164,996
Theobroma cacao	3376	3979	6,027,724	226,779	114,082	80,088
Phoenix dactylifera	8978	10,334	7,341,396	716,454	218,488	177,248
Amborella trichopoda	11,702	13,558	10,008,594	787,796	234,856	120,412
Beta vulgaris	6458	7804	7,904,182	483,300	199,616	103,224
Sesamum indicum	3412	4135	4,915,092	247,403	113,511	78,634
Eucalyptus grandis	8364	9943	9,894,486	849,552	307,001	174,810
Prunus persica	3116	4351	3,505,137	237,185	90,631	67,280
Solanum lycopersicum	15,086	18,005	12,249,900	596,412	255,692	124,564
Oryza sativa	10,042	11,855	4,881,316	595,046	138,534	171,045
Phyllostachys edulis	51,097	58,916	23,112,695	3,233,281	734,959	707,912
Sorghum bicolor	21,528	23,978	8,200,062	1,217,504	301,472	329,922
Setaria italica	15,347	17,861	4,683,693	757,165	145,882	225,478
Zea mays	85,902	2797	21,096,385	4,784,940	807,008	886,527

ISSN: 1746-4811