Fig. 2From: In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sectionsImages of samples of C. × morifolium infected with P. horiana prepared by ISH (Red scale bar = 100 μm). a Samples processed with (top) and without (bottom) a proteinase K digestion. b Samples processed using four different proteinase K concentrations. Purple staining of the tissue indicates hybridization signal generated by HRP reacting with purple substrateBack to article page