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Fig. 7 | Plant Methods

Fig. 7

From: RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation

Fig. 7

Root and nodule growth dynamic obtained by digital imaging analysis. Images of pea plant (kayanne genotype) grown in RhizoTube without soil mineral nitrogen in experiment 3 were realized using RhizoCab at resolution of 600 ppi and RVB light. Examples of nodulated roots images (with a focus in the yellow colored square) taken at 309, 464 and 597 degree-days are shown in a, b and c respectively. Image segmentation allows to obtain the pixels numbers for roots and nodules. These values are then converted in biomass (grams) using a calibration curves (data not shown). As such, nodule biomass (open circles) and root biomass (closed circles) can be dynamically estimated during the growth cycle (d)

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