Fig. 7From: RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validationRoot and nodule growth dynamic obtained by digital imaging analysis. Images of pea plant (kayanne genotype) grown in RhizoTube without soil mineral nitrogen in experiment 3 were realized using RhizoCab at resolution of 600 ppi and RVB light. Examples of nodulated roots images (with a focus in the yellow colored square) taken at 309, 464 and 597 degree-days are shown in a, b and c respectively. Image segmentation allows to obtain the pixels numbers for roots and nodules. These values are then converted in biomass (grams) using a calibration curves (data not shown). As such, nodule biomass (open circles) and root biomass (closed circles) can be dynamically estimated during the growth cycle (d)Back to article page