Fig. 2

Detection of the sense strand in v.3 unigenes by hybridization to the 244k microarray. The signal intensities of probes representing each contig in both orientations were compared, and the probe with the stronger signal was defined as the sense one. a, b Probes selected for the Hyb-high-confidency set, with an intensity ratio ≥4 and an identical (a) or reverse complement (b) orientation relative to the v.3 unigene sequence; c, d probes selected for the Hyb-low-confidency set, with an intensity ratio ≥2, but less than 4 and an identical (c) or reverse complement (d) orientation relative to the v.3 unigene sequence. Probes identified as sense are marked in orange/red; the antisense pairs are in black