Table 1 Details of hormone/antibiotic stock preparation
From: Protocol: a method to study the direct reprogramming of lateral root primordia to fertile shoots
Antibiotic/hormone | Stock concentration | Solvent | Concentration in media | Amount to be added for 50 ml medium (µl) | Amount to be added for 1 l medium (ml) |
|---|---|---|---|---|---|
Ampicillin | 100 mg/ml | 40 % ethanol | 100 µg/ml | 50 | 1 |
NAA | 1.86 mg/ml | DMSO | 10 µM | 50 | 1 |
2iP | 10 mM | DMSO | 10 µM | 50 | 1 |
Trans zeatin | 10 mM | DMSO | 10 µM | 50 | 1 |