Fig. 6

The 450 bp deletion in the AG 2nd intron results in reduction of AG expression without interfering the splicing of the gene. a AG expression in wild type and mutant was detected by three pairs of primers shown in the upper scheme. The expression data represents three biological replicates and three technical replicates. Data were shown as mean ± SD. b Agarose gel pictures of products of two rounds of PCR, a reverse transcription PCR using oligodT primer as one of the primers followed by a nested PCR using a more internal reverse primer. c Sequencing results of the products from the second round of PCR in wild type and mutant